diff --git a/cypress/e2e/spaces/spaces.spec.ts b/cypress/e2e/spaces/spaces.spec.ts
index c27d6e5ad6..6f59e74401 100644
--- a/cypress/e2e/spaces/spaces.spec.ts
+++ b/cypress/e2e/spaces/spaces.spec.ts
@@ -294,6 +294,11 @@ describe("Spaces", () => {
                 "nested-interactive": {
                     enabled: false,
                 },
+                // Disable this check as it wrongly triggers on the room list container which has
+                // roving tab index elements with automatic scrolling
+                "scrollable-region-focusable": {
+                    enabled: false,
+                },
             },
         };
         cy.checkA11y(undefined, axeOptions);